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Image Search Results
Journal: PLoS ONE
Article Title: Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells
doi: 10.1371/journal.pone.0162111
Figure Lengend Snippet: ( A ) Representative flow cytometry profiles of CD8 and CD44 expression on TCRβ + T cells in spleens mice 14d after tamoxifen or vehicle treatment. ( B ) Proportions of CD8 + T cells expressing high levels of CD44 (CD44 hi ) in spleens of mice at the times indicated after tamoxifen or vehicle treatment. ( C ) Proportions of naïve (CD44 - CD62L + ), central memory (CD44 + CD62L + ) and effector memory (CD44 + CD62L - ) CD3 + T cells in spleens of mice 50d (top panels) or 180d (bottom) after tamoxifen or vehicle treatment (m, moribund). Mean ± SD is shown with * p<0.05 for comparison of γ S1S3 (moribund) with all genotypes at day 50 except γ S1 (non-moribund day 50). ** p<0.05 for comparison of γ S1 (moribund) with all genotypes at day 180 except γ S1 (non-moribund, day 180), one-way ANOVA with Tukey’s multiple comparisons test, n = 7–20 mice per group.
Article Snippet: CD8 + cells were enriched from spleen cell preparations via positive selection using anti-CD8a (Ly-2) microbeads (
Techniques: Flow Cytometry, Expressing, Comparison
Journal: PLoS ONE
Article Title: Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells
doi: 10.1371/journal.pone.0162111
Figure Lengend Snippet: ( A ) Representative flow cytometry profiles showing expression of activation and homing markers as indicated on CD8 + CD44 high cells from spleens of mice 14d after tamoxifen or vehicle treatment. ( B ) CD8 + T cells were purified from spleens of mice 14d following tamoxifen or vehicle treatment. The cells were stimulated for 72 hours in culture in the presence of IL-15 or IL-2 plus anti-CD3/CD28. Expression of CD25, CD69, CD44 and CD62L before and after culture in a representative experiment is shown.
Article Snippet: CD8 + cells were enriched from spleen cell preparations via positive selection using anti-CD8a (Ly-2) microbeads (
Techniques: Flow Cytometry, Expressing, Activation Assay, Purification
Journal: PLoS ONE
Article Title: Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells
doi: 10.1371/journal.pone.0162111
Figure Lengend Snippet: Concentrations of cytokines/chemokines in supernatant of CD8 + T cells 48hr after stimulation with IL-2 plus anti-CD3/CD28 ( A ) or IL-15 ( B ). Mean ± SD is shown. CD45.2 + CD3 + CD8 + T cells were flow-sorted from spleens of mice 14d after tamoxifen or vehicle treatment. Mean ± SD is shown with * p<0.05 for comparison of γ S1S3 with TAM- and S3 . ** p<0.05 for comparison of γ S1 with TAM- and S3 , one-way ANOVA with Tukey’s multiple comparisons test, n = 6–7 mice per group.
Article Snippet: CD8 + cells were enriched from spleen cell preparations via positive selection using anti-CD8a (Ly-2) microbeads (
Techniques: Comparison